Engineered Metabolism for Chemical Production from One-carbon Substrates 一碳物质的化工与生物利用技术
讲座名称：
Engineered Metabolism for Chemical Production from One-carbon Substrates 一碳物质的化工与生物利用技术
讲座时间：
2019-03-18
讲座人：
Ramon Gonzale
形式：
校区：
兴庆校区
实践学分：
讲座内容：
讲座题目：Engineered Metabolism for Chemical Production from One-carbon Substrates一碳物质的化工与生物利用技术
讲座时间：3月18日 上午10：00
讲座地点：教学二区化工学院办公楼504会议室
讲座人：Ramon Gonzale
讲座简介：
One-carbon (C1) compounds, including carbon dioxide, carbon monoxide, formate, and methane, are attractive feedstocks for fuel and chemical production due to their availability and sustainability. However, the efficient and economical utilization of these feedstocks can be challenging for traditional chemical processes due, in part, to their diffuse nature. In this talk, we will discuss our recent efforts to engineer and implement biological C1 utilization for chemical production. We have leveraged the existing C1-utilization pathways of native methanotrophMethylomicrobium buryatensefor the production of industrially relevant products such as lactate. We also engineered a synthetic metabolic pathway for C1 conversion to multi-carbon products that is distinctive from and orthogonal to any known metabolic network. This C1 elongation pathway is enabled by our discovery that an enzyme involved in mammalian α-oxidation (2-hydroxyacyl-CoA lyase, HACL) can catalyze the condensation of formyl-CoA, an activated C1 molecule, with aldehydes of varying chain lengths. Our work establishes the potential for biotechnological applications of HACL, which includes both bioconversion of C1 feedstocks as well as synthetic methylotrophy and autotrophy.
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