Jurgen Popp教授讲座（二） Raman Spectroscopy a powerful approach towards labelfree clinical diagnostics
讲座名称：
Jurgen Popp教授讲座（二） Raman Spectroscopy a powerful approach towards labelfree clinical diagnostics
讲座时间：
2013-10-23
讲座人：
Jurgen Popp
形式：
校区：
兴庆校区
实践学分：
讲座内容：
讲座题目 Raman Spectroscopy a powerful approach towards labelfree clinical
diagnostics
主讲人 Jurgen Popp
讲座时间/地点 10月23日 15:00～16:30 中1-3113
讲座领域 光学遥感探测、光电子学、信息图像处理
内容简介 Here, we briefly describe some of our latest results concerning the application of linear and nonlinear Raman microspectroscopy to characterize a broad range of different tissue sections (biopsy specimens) for biomedical diagnosis. We will start with showing that the processing of the chemically specific tissue Raman-maps via mathematical approaches for a spectral analysis and classification enables an objective evaluation of the tissue samples for an early
disease diagnosis like e.g. cancer or inflammatory bowel disease. Besides these ex-vivo tissues Raman studies (i.e. Raman pathology) first steps towards in-vivo Raman spectroscopy that is Raman endospectroscopy will be presented. By doing so novel Raman fiber probes for an intraoperative monitoring of the artheriosclerotic plaque in living rabbits will be presented.
The low Raman scattering cross section results in long acquisition times limiting the recording of Raman images of large tissue areas and thus, clinical applications. The acquisition times can be reduced by utilizing non-linear Raman approaches like CARS (coherent anti-Stokes Raman scattering) and allows recording Raman images of single characteristic Raman bands in real time. It will be shown, that the joint use of linear Raman microspectroscopy and CARS microscopy allows for complementary characterization of the type and chemical composition of the tissue samples. While linear Raman microspectroscopy is used to obtain the information on critical Raman marker bands at selected spatial position within the sample, CARS microscopy is focusing on fast image generation using the previously defined Raman marker bands. In order to improve the diagnostic result CARS microscopy can be easily combined with second harmonic generation (SHG) and two-photon fluorescence (TPF) microscopy. SHG and TPF highlightmorphological / structural features by displaying collagen structures (SHG) and the spatialdistribution of autofluorophores like e.g. NAD(P)H, flavines, elastine etc. Overall, we will present the development of a compact CARS/SHG/TPF multimodal nonlinear microscope in combination with novel fiber laser sources for use in clinics. The diagnostics potential of this compact multimodal microscope as compared to conventional histopathological images has been demonstrated for the examples of cancer and atherosclerosis. These examples show the great potential of multimodal imaging to complement established clinical pathological diagnostic tools.
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