Interfacing DNA with metal ions, metal oxides, and metal nanoparticles
讲座名称：
Interfacing DNA with metal ions, metal oxides, and metal nanoparticles
讲座时间：
2017-05-16
讲座人：
刘珏文
形式：
校区：
兴庆校区
实践学分：
讲座内容：
报告题目： Interfacing DNA with metal ions, metal oxides, and metal nanoparticles
报告时间： 2017年5月16日 下午16:00-17:30
报告地点： 基础学科大楼B249
报 告 人： 加拿大滑铁卢大学-刘珏文教授
报告摘要:
DNA is a polyanion with strong affinity towards metal containing species. We are interested in studying the interaction between DNA and metal for analytical applications. This talk will cover three aspects. 1) In vitro selection of metal-specific DNAzymes. DNAzymes are DNA-based catalysts and we focus on RNA-cleaving DNAzymes. Using lanthanide ions as cofactors, we isolated a suite of new DNAzymes that can selectively detect rare-earth metals. By using phosphorothioate modified DNAzymes, we developed new probes for a few toxic heavy metal ions. 2) The adsorption of DNA by various metal oxides were studied, where the phosphate backbone of DNA is shown to be critical for the adsorption reaction. Using fluorescently labeled DNA, highly sensitive detection of arsenate and hydrogen peroxide was achieved. 3) DNA adsorption by gold nanoparticles is studied as a function of salt concentration and pH. Based on this study, we developed an ultrasensitive method for functionalizing gold surface with DNA.
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